lambda ladder pfge size standard  (New England Biolabs)

Journal: BMC Veterinary Research

Article Title: Faecal shedding and strain diversity of Listeria monocytogenes in healthy ruminants and swine in Northern Spain

doi: 10.1186/1746-6148-5-2

Figure Lengend Snippet: Dendrogram (UPGMA) of the Apa I PFGE patterns of L. monocytogenes isolates from 6 different herds (A-G as in Table 2) . The isolates have been coded according to sample type of origin: P, pool of faeces; I, individual faecal sample.

Article Snippet: Fragments were separated by electrophoresis in a CHEF-DRII system (BioRad) at a constant temperature of 14°C during 5 h using an initial switch time of 15 s and a final switch time of 35 s and a further 15 h with switch times of 2–20 s. Gels were normalised by alignment with the Lambda Ladder PFGE size standard (New England Biolabs, MA, USA) and Salmonella Braenderup control strain H9812 digested with Xba I [ ].

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Standardization and Interlaboratory Reproducibility Assessment of Pulsed-Field Gel Electrophoresis-Generated Fingerprints of Acinetobacter baumannii

doi: 10.1128/jcm.43.9.4328-4335.2005

Figure Lengend Snippet: FIG. 1. PFGE profiles of 20 A. baumannii isolates (lanes 1 to 20) used in study phase I digested with ApaI. The profiles were generated in laboratory B. Isolates represent both outbreak-related and sporadic strains. The participating laboratories were blinded to the identities of the isolates. Lanes M, bacteriophage lambda ladder; lanes R, A. baumannii COL 20820, which was the external reference standard used for normalization of the gels and which was run on every fourth to fifth lane. See Table 1 for details about the strains.

Article Snippet: A slice of a bacteriophage lambda ladder PFGE marker (CHEF DNA size standard; catalog no. 170-3035; Bio-Rad Laboratories) was loaded into lane 1; and reference strain COL 20820 was loaded into lanes 2, 6, 11, and 15 to allow later normalization of the electrophoretic patterns across the gel.

Techniques: Generated

Journal: Journal of Clinical Microbiology

Article Title: Standardization and Interlaboratory Reproducibility Assessment of Pulsed-Field Gel Electrophoresis-Generated Fingerprints of Acinetobacter baumannii

doi: 10.1128/jcm.43.9.4328-4335.2005

Figure Lengend Snippet: FIG. 2. PFGE profiles of representative A. baumannii isolates (lanes 1 to 5) digested with ApaI and obtained by using a highly standardized protocol for PFGE, as performed in laboratories A to C (panels A to C, respectively). Lanes R, A. baumannii COL 20820, which was the external reference standard. See Table 1 for details about the strains.

Article Snippet: A slice of a bacteriophage lambda ladder PFGE marker (CHEF DNA size standard; catalog no. 170-3035; Bio-Rad Laboratories) was loaded into lane 1; and reference strain COL 20820 was loaded into lanes 2, 6, 11, and 15 to allow later normalization of the electrophoretic patterns across the gel.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Standardization and Interlaboratory Reproducibility Assessment of Pulsed-Field Gel Electrophoresis-Generated Fingerprints of Acinetobacter baumannii

doi: 10.1128/jcm.43.9.4328-4335.2005

Figure Lengend Snippet: FIG. 3. Computer-assisted analysis of PFGE patterns generated at three different laboratories in study phase I confirmed the interpreta- tions obtained at the local laboratories and showed similarity values of 87% for the corresponding isolates processed at different laboratories.

Article Snippet: A slice of a bacteriophage lambda ladder PFGE marker (CHEF DNA size standard; catalog no. 170-3035; Bio-Rad Laboratories) was loaded into lane 1; and reference strain COL 20820 was loaded into lanes 2, 6, 11, and 15 to allow later normalization of the electrophoretic patterns across the gel.

Techniques: Generated

Journal: Journal of Clinical Microbiology

Article Title: Standardization and Interlaboratory Reproducibility Assessment of Pulsed-Field Gel Electrophoresis-Generated Fingerprints of Acinetobacter baumannii

doi: 10.1128/jcm.43.9.4328-4335.2005

Figure Lengend Snippet: FIG. 4. Gel images of PFGE patterns of 30 A. baumannii isolates (lanes 21 to 50), to whose identities the participating laboratories were blinded, from 10 hospital outbreaks, represented by 3 isolates each. Each laboratory (laboratories A to C; panels A to C, respectively) therefore processed 10 isolates from the 10 outbreaks; laboratory A, strains 41 to 50; laboratory B, strains 21 to 30; and laboratory C, strains 31 to 40. Lanes M, bacteriophage lambda ladder; lanes R, A. bauman- nii COL 20820, which was the reference standard and which was run on every fourth to fifth lane. See Table 2 and Fig. 5 for details about the strains.

Article Snippet: A slice of a bacteriophage lambda ladder PFGE marker (CHEF DNA size standard; catalog no. 170-3035; Bio-Rad Laboratories) was loaded into lane 1; and reference strain COL 20820 was loaded into lanes 2, 6, 11, and 15 to allow later normalization of the electrophoretic patterns across the gel.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Standardization and Interlaboratory Reproducibility Assessment of Pulsed-Field Gel Electrophoresis-Generated Fingerprints of Acinetobacter baumannii

doi: 10.1128/jcm.43.9.4328-4335.2005

Figure Lengend Snippet: FIG. 5. Computer-assisted analysis of PFGE patterns generated at three different laboratories in study phase II correctly identified 3 isolates from each of 10 outbreaks among the isolates investigated.

Article Snippet: A slice of a bacteriophage lambda ladder PFGE marker (CHEF DNA size standard; catalog no. 170-3035; Bio-Rad Laboratories) was loaded into lane 1; and reference strain COL 20820 was loaded into lanes 2, 6, 11, and 15 to allow later normalization of the electrophoretic patterns across the gel.

Techniques: Generated